| 2026-06-27 | Queen of the South v GAP Connah S Quay FC | 0–1 | Club Friendlies |
| 2026-07-04 | Queen of the South v Crusaders FC | 1–2 | Club Friendlies |
| 2026-07-11 | Queen of the South v Kelty Hearts | 1–1 | Group Stage |
| 2026-07-18 | Brora Rangers v Queen of the South | 0–3 | Group Stage |
| 2026-07-22 | Queen of the South v Aberdeen Football Club | 1–4 | Group Stage |
| 2026-07-25 | Queen's Park v Queen of the South | 3–1 | Group Stage |
| 2026-08-01 | East Kilbride v Queen of the South | 5–1 | Regular Season - 1 |
| 2026-08-08 | Queen of the South v Peterhead | 0–1 | Regular Season - 2 |
| 2026-08-11 | Queen of the South v Cumbernauld Colts | 4–0 | Group Stage - 1 |
| 2026-08-22 | Queen of the South v Alloa Athletic | 0–2 | Regular Season - 4 |
| 2026-08-25 | Queen of the South v Hibernian U21 | 1–0 | Group Stage - 2 |
| 2026-08-29 | Montrose v Queen of the South | 2–1 | Regular Season - 5 |
| 2026-09-05 | Queen of the South v Airdrie United | 2–1 | Regular Season - 6 |
| 2026-09-08 | Edinburgh City v Queen of the South | 1–1 | Group Stage - 3 |
| 2026-09-12 | Queen of the South v East Fife | 1–1 | Regular Season - 7 |
| 2026-09-15 | Ross County FC v Queen of the South | 8–0 | Regular Season - 3 |
| 2026-09-19 | Hamilton Academical FC v Queen of the South | 4–1 | Regular Season - 8 |
| 2026-09-22 | Queen of the South v Annan Athletic | 1–1 | Group Stage - 4 |
| 2026-09-26 | Queen of the South v Rangers U21 | 4–2 | Group Stage - 5 |
| 2026-10-03 | Queen of the South v Cove Rangers | 4–3 | Regular Season - 9 |
| 2026-10-10 | Peterhead v Queen of the South | 0–1 | Regular Season - 10 |
| 2026-10-13 | Berwick Rangers v Queen of the South | 1–0 | Group Stage - 6 |
| 2026-10-17 | Queen of the South v Ross County FC | — | Regular Season - 11 |
