| 1 | Cristiano Ronaldo | Manchester United Football Club | Real Madrid Club de Fútbol | €94M | 6 July 2009 |
| 2 | Karim Benzema | Olympique Lyonnais | Real Madrid Club de Fútbol | €35M | 9 July 2009 |
| 3 | Dmytro Chygrynskyi | FC Shakhtar Donetsk | Futbol Club Barcelona | €25M | 31 August 2009 |
| 4 | Álvaro Negredo | Real Madrid Club de Fútbol | Sevilla Fútbol Club S.A.D. | €15M | 21 August 2009 |
| 5 | Raúl Albiol | Valencia Club de Fútbol S. A. D. | Real Madrid Club de Fútbol | €15M | 1 July 2009 |
| 6 | Leonardo Bonucci | Genoa Cricket and Football Club | — | €10.5M | 1 August 2009 |
| 7 | Lukas Podolski | FC Bayern München | 1. FC Köln | €10M | 1 July 2009 |
| 8 | Dejan Lovren | Dinamo Zagreb | Olympique Lyonnais | €9M | 13 January 2010 |
| 9 | Eduardo Salvio | Club Atlético Lanús | Club Atlético de Madrid S.A.D. | €8M | 11 January 2010 |
| 10 | Adem Ljajic | FK Partizan | Associazione Calcio Fiorentina | €6.8M | 13 January 2010 |
| 11 | Iván Marcano | — | Villarreal Club de Fútbol S.A.D. | €6M | 3 July 2009 |
| 12 | Kyle Walker | Sheffield Utd | Tottenham Hotspur Football Club | €5.9M | 22 July 2009 |
| 13 | Lorenzo De Silvestri | Società Sportiva Lazio S.p.A. | Associazione Calcio Fiorentina | €5.5M | 26 August 2009 |
| 14 | İsmail Köybaşı | Gaziantepspor (- 2020) | Beşiktaş Jimnastik Kulübü | €5.5M | 21 July 2009 |
| 15 | Radamel Falcao | Club Atlético River Plate | Futebol Clube do Porto | €5.4M | 15 July 2009 |
| 16 | James McCarthy | Hamilton Academical FC | WIG | €5.1M | 16 July 2009 |
| 17 | Álvaro Negredo | Almeria | Real Madrid Club de Fútbol | €5M | 1 July 2009 |
| 18 | Mats Hummels | FC Bayern München | Borussia Dortmund | €4.2M | 1 July 2009 |
| 19 | Jan Oblak | — | Benfica | €4M | 4 January 2010 |
| 20 | Leonardo Bonucci | Inter | Genoa Cricket and Football Club | €4M | 1 July 2009 |
| 21 | Asmir Begović | — | Stoke City | €3.8M | 1 February 2010 |
| 22 | Antonio Mirante | Sampdoria | Parma Calcio 1913 | €3.6M | 1 July 2009 |
| 23 | Victor Moses | Crystal Palace Football Club | WIG | €3M | 31 January 2010 |
| 24 | Dani Parejo | Real Madrid Club de Fútbol | Getafe Club de Fútbol S. A. D. Team Dubai | €3M | 25 July 2009 |
| 25 | Lewis Holtby | — | FC Schalke 04 | €3M | 7 July 2009 |
| 26 | Ricky van Wolfswinkel | Vitesse | Football Club Utrecht | €2.8M | 1 July 2009 |
| 27 | Chung-yong Lee | Football Club Seoul | — | €2.5M | 14 August 2009 |
| 28 | Timothée Kolodziejczak | Racing Club de Lens | Olympique Lyonnais | €2.5M | 1 July 2009 |
| 29 | Sung-yueng Ki | Football Club Seoul | The Celtic Football Club | €2.4M | 1 January 2010 |
| 30 | Danijel Aleksic | Fudbalski Klub Vojvodina | Genoa Cricket and Football Club | €2.3M | 19 January 2010 |
| 31 | Alex Sandro | Clube Atlético Paranaense | — | €2.2M | 1 February 2010 |
| 32 | Nenad Tomovic | FK Crvena Zvezda | Genoa Cricket and Football Club | €2.2M | 1 July 2009 |
| 33 | Maicon | Cruzeiro | Futebol Clube do Porto | €2.2M | 1 July 2009 |
| 34 | Teófilo Gutiérrez | Junior FC | Trabzonspor Kulübü | €2.1M | 19 January 2010 |
| 35 | Adrián Ramos | America de Cali | Hertha BSC | €2M | 31 August 2009 |
| 36 | Nemanja Matić | — | Chelsea Football Club | €1.8M | 18 August 2009 |
| 37 | Joselu | Real Club Celta de Vigo S. A. D. | — | €1.5M | 30 August 2009 |
| 38 | Leandro Cabrera | — | Club Atlético de Madrid S.A.D. | €1.5M | 16 July 2009 |
| 39 | José Fonte | Crystal Palace Football Club | Southampton FC | €1.4M | 9 January 2010 |
| 40 | Lukas Marecek | — | Anderlecht | €1.2M | 1 January 2010 |
| 41 | Eduardo Vargas | — | — | €1M | 6 January 2010 |
| 42 | Marco Reus | — | Borussia Mönchengladbach | €1M | 1 July 2009 |
| 43 | Luuk de Jong | De Graafschap Doetinchem | Football Club Twente | €900k | 1 July 2009 |
| 44 | Matej Vydra | — | Baník Ostrava | €800k | 21 January 2010 |
| 45 | Tomas Pekhart | Tottenham Hotspur Football Club | FK Jablonec a.s. | €800k | 11 January 2010 |
| 46 | Gianluca Pegolo | Genoa Cricket and Football Club | Siena FC | €800k | 1 July 2009 |
| 47 | Masato Morishige | — | FC Tokyo | €750k | 4 January 2010 |
| 48 | Raheem Sterling | — | — | €700k | 16 February 2010 |
| 49 | Bibars Natcho | — | Rubin | €650k | 8 March 2010 |
| 50 | Dayro Moreno | FCSB | Once Caldas | €600k | 1 January 2010 |